Both the Martin Li and Mr. Negative personas are the result of experimental drug tests done by Maggia chemist Simon Marshall, the same tests that powered Cloak and Dagger (namely Cloak's association with the Darkforce).
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During the first story of the Brand New Day, Mister Negative first comes into conflict with Spider-Man when he makes a power play toward taking control of New York's criminal underworld by attempting to wipe out all existing members of the Karnelli and Maggia crime families using a DNA specific bioweapon called the "Devil's Breath".
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Created by Dan Slott, Steve McNiven, and Phil Jimenez, Mister Negative first appeared in a cameo in Amazing Spider-Man: Swing Shift (May 2007), a Free Comic Book Day issue set after the events of the Spider-Man: One More Day storyline.
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Mister Negative's first full appearance came in The Amazing Spider-Man #546 (Jan 2008); the start of the "Brand New Day" storyline.
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During The Gauntlet storyline, Mister Negative corrupts Spider-Man's Aunt May when she walks in on him punishing an Inner Demon.
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Art by Pasqual Ferry | Renninger's negative-result gedanken experiment | Proving a negative | Negative keyword | Negative FX |