Following shortly after the success of the Mocama missions, the Spanish established missions among the Agua Fresca (Eastern Utina or Freshwater Timucua) along the middle St. Johns River, from roughly present-day Palatka south to Lake George.
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A Spanish mission, Santa Isabel de Utinahica, was established in the chief town of the Utinahica on the Altamaha River, near the present site of Jacksonville, Georgia, in the first half of the 17th century.