3,3’,5,5’-Tetramethylbenzidine or TMB is a chromogenic substrate used in staining procedures in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays (ELISA).
His research during this time focused on biological and chemical assays on vitamins, proteins, and other nutrient; pharmaceutical vitamin fortification, stabilization, and availability; toxicology and safety evaluation of food additives, drugs, pesticides, and related chemicals; and the scientific aspects of food law and regulations.
A study by The American Journal of Clinical Nutrition that has been cited to support D'Adamo's theories reported that the edible parts of 29 of 88 foods tested, including common salad ingredients, fresh fruits, roasted nuts, and processed cereals, were found to possess significant lectin-like activity (as assessed by hemagglutination and bacterial agglutination assays).
speciosa to be a synonym of C. indica L., however, Tanaka's DNA assays demonstrate that the C. indica complex can be clearly distinguished from other taxa, as a result he recognises this as a separate species.
According to a research by A. Pieroni, V. Janiak, C. M. Dürr, S. Lüdeke, E. Trachsel and M. Heinrich (2002) in the Centre for Pharmacognosy and Phytotherapy (The School of Pharmacy, University of London) "the antioxidant activity of the young whorls of Centaurea calcitrapa, both in the DPPH and in the lipid peroxidation inhibition assays, are very interesting and the species should be investigated phytochemically and biochemically focusing on these
ChIP-on-chip assays, which is based on the ability of commercially prepared antibodies to bind to DNA methylation-associated proteins like MeCP2.
While performing molecular assays involving use of antibodies such as in the Western blot, immunohistochemistry, and ELISA, one should carefully choose antibodies that recognize linear or conformational epitopes.
Common crosslinkers for this application include the non-cleavable NHS-ester cross-linker, bissulfosuccinimidyl suberate (BS3); a cleavable version of BS3, dithiobis(sulfosuccinimidyl propionate) (DTSSP); and the imidoester cross-linker dimethyl dithiobispropionimidate (DTBP) that is popular for fixing interactions in ChIP assays.
Using liquid chromotography in conjunction with several biological assays, PDF, was also isolated in the insect Leucophaea maderae, a cockroach.
This offering enables a single solution for managing clinical study data, treatment groups data, subject data, sample data and assay data from diverse assays such as Immunohistochemistry, Western Blot, PCR, etc.
There are many variations, or types of ELISA assays but they can generally be classified as either indirect, competitive, sandwich or reverse.