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The methyl ester of calostomal was subjected to NMR spectroscopy and was identified as all-trans-16-oxohexadeca-2,4,6,8,10,12,14-heptaenoic acid.
These folate-dependent biosynthetic reactions include the transfer of methyl groups from 5-methyltetrahydrofolate to homocysteine to form formyl groups from 10-formyltetrahydrofolate to N-formylmethionine in initiator tRNAs.
Petunidin could form in the exocarp of fruits from delphinidin, with an anthocyanin flavonoid O-methyltransferase (Catechol-O-methyl transferase) catalyzing the B-ring methylation and S-Adenosyl-L-methyl-3H methionine being the methyl group donor.