She is noted for discovering, with her then-husband Kenneth C. Brugger, the location of the overwintering sites of the Monarch butterfly, Danaus plexippus L.
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Trail and Brugger's search for the butterflies is dramatized in the IMAX film Flight of the Butterflies.
On January 9, 1975, Kenneth C. Brugger and his wife Catalina Trail (then known as Cathy Aguado) finally located the first known wintering refuge on a mountaintop in Michoacán, Mexico, more than 4,000 kilometers from the starting point of their migration.
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The five minute video captures the new Trans Catalina Trail for the Catalina Island Conservancy.