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Most engine designs that pre-dated the Otto engine (and Clerk engine), such as those of de Rivaz, the Niépce brothers, Jean Joseph Etienne Lenoir, Samuel Morey, and others, did use two stroke engines, which were "natural" in the times of steam engine.
1807 - Franco/Swiss engineer François Isaac de Rivaz built the De Rivaz engine, powered by the internal combustion of hydrogen and oxygen mixture and used it to power a wheeled vehicle.