X-Nico

21 unusual facts about ELISA#"Indirect" ELISA


3,3',5,5'-Tetramethylbenzidine

3,3’,5,5’-Tetramethylbenzidine or TMB is a chromogenic substrate used in staining procedures in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays (ELISA).

ABTS

A common use for it is in the enzyme-linked immunosorbent assay (ELISA) to detect for binding of molecules to each other.

Bioanalysis

The most frequently used techniques are: liquid chromatography coupled with tandem mass spectrometry (LC–MS/MS) for 'small' molecules and enzyme-linked immunosorbent assay (ELISA) for macromolecules

Biuret test

ELISA - Yet another method applied even today in clinical services.

Elisa

Evolved Laser Interferometer Space Antenna (eLISA), a proposed configuration for a space based gravitational wave detector

ELISA or Enzyme-Linked Immunosorbent Assay, a biochemical technique

Equine drug testing

Around that time, highly sensitive ELISA testing of horse urine was introduced to racing regulators by a group at the University of Kentucky.

Fast protein liquid chromatography

The range of purity required can be from that required for basic analysis (SDS-PAGE or ELISA, for example), with only bulk impurities removed, to pure enough for structural analysis (NMR or X-ray crystallography), approaching >99% target molecule.

Granzyme

Granzyme secretion can be detected and measured using Western Blot or ELISA techniques.

Hepatitis A vaccine

Each adult dose contains 1440 ELISA units of viral antigen adsorbed on aluminium hydroxide (0.5 mg Al).

In vitro toxicology

ELISA kits can be used to examine up and down regulation of proinflammatory mediators such as cytokines (IL-1, TNF alpha, PGE2)....

Infectious pancreatic necrosis

Diagnostic methods for the detection of the disease include: characteristic histological pancreatic lesion, PCR, indirect fluorescent antibody testing, ELISA, and virus culture.

Jorge Benach

Benach and pathology colleague Marc Golightly developed the critical laboratory test to detect the presence of antibodies to Borrelia burgdorferi, an ELISA test that became the original “gold standard” for Lyme diagnosis.

Legionella pneumophila

ELISA and microagglutination tests have also been successfully applied.

Linear epitope

While performing molecular assays involving use of antibodies such as in the Western blot, immunohistochemistry, and ELISA, one should carefully choose antibodies that recognize linear or conformational epitopes.

Streptococcus suis

The bacterium can be isolated from various body fluids, and serological testing with an ELISA can also be performed.

Tapinoma sessile

They labeled sucrose with Immunoglobin G (IgG) proteins, and then identified them using an enzyme-linked immunosorbent assay (ELISA) to track the movement of food.

Tick-borne encephalitis

Serological testing can also be performed with an ELISA.

Toxocaridae

Direct identification of larvae in human disease is not feasible, so the diagnosis relies on history supported by serologic ELISA assay.

Virus quantification

ELISA is a more modern variation of a protein assay that utilizes a specific antibody linked to an enzyme to detect the presence of an unknown amount of antigen (i.e. virus) in a sample.

There are many variations, or types of ELISA assays but they can generally be classified as either indirect, competitive, sandwich or reverse.


Alcide Nunez

His parents were Victor Nunez and Elisa Nunez Chalaire and were of Isleño and Louisiana French descent respectively.

Almeno tu nell'universo

A cover of the song was recorded by Italian singer Elisa in 2003 for the official soundtrack of the movie Remember Me, My Love, directed by Gabriele Muccino.

Anna Bonitatibus

She has recorded several times under the direction of Alan Curtis; duets by Alessandro Scarlatti with Patrizia Ciofi, an album of Haydn arias L'infedeltà costante, and Handel operas; Elisa in Tolomeo, and the castrato role of Ulysses in Deidamia.

APA Task Force on Deceptive and Indirect Methods of Persuasion and Control

Cults and large group awareness trainings have generated considerable controversy because of their widespread use of deceptive and indirect techniques of persuasion and control.

Boris Palmer

He withdrew his candidacy before the second round ballot with an indirect recommendation that his followers should vote for the incumbent mayor Wolfgang Schuster on condition that Schuster would hold a referendum on the controversial project Stuttgart 21, which Palmer is opposed to, if costs would increase significantly.

Chem-Mod

Spencer Abraham, the former Secretary of Energy, is listed as an advisor and indirect equity holder of Chem-Mod.

CIA activities in Iran

Beginning in August 1984, a small group within the US government, in the Iran-Contra affair, arranged for the indirect transfer of arms to Iran, as a means of circumventing the Boland Amendments that were intended, in part, to prevent the expenditure of US funds to support the Nicaraguan Contras.

CIA and Contras cocaine trafficking in the US

Then on December 18, 1997, stories in the Washington Post and New York Times appeared, stating that Hitz had found "no direct or indirect" links between the CIA and cocaine traffickers, despite the reporters never seeing the report.

Cinta 7 Susun

Cast is 7icons, Mischa Chandrawinata, Baim Wong, Raya Kohandi, Devi Noviaty, Gege Elisa, Shandy Ishabella, Gunawan Sudradjat, Cut Memey, Rico Tampatty, Minati Atmanegara, Mario Maulana as well as other supporting cast as well as some cameo actors who have often appeared on Indonesian movie or television series.

Death of Zahra Baker

Apparently, Elisa told her attorney that Zahra's prosthetic leg was left in a dumpster that she and Adam had disposed of at Fox Ridge Apartments in Hickory.

Emilio Bigi

Emilio Bigi was born in Asunción, Paraguay, on July 1, 1910, son of Elisa Bigi, from the Italian collectivity in this country.

Ethan of Athos

Alternatively, it may be an indirect tribute to the actor Oliver Reed, whom Bujold is known to admire (she has cited him as a model for Miles' father Aral Vorkosigan); Reed played the role of Athos in the 1973 film version of The Three Musketeers.

Forward Operating Base Salerno

Located in the southeastern province of Khost, Afghanistan, near the city of Khost, FOB Salerno was nicknamed "Rocket City" for the numerous rocket and mortar indirect fire attacks targeting the base over the ten years that US forces occupied to post.

Four-dimensional space

The retina of the eye is also a two-dimensional array of receptors but the brain is able to perceive the nature of three-dimensional objects by inference from indirect information (such as shading, foreshortening, binocular vision, etc.).

Fraser Frisell

In memory of Frisell's daughter Élisa, who died at Passy in 1832, Chateaubriand, while in prison on charge of participation in the Duchesse de Berry's attempt to overthrow the Orléanist régime, composed the touching stanzas, Jeune Fille et Jeune Fleur, and portions of the affectionate correspondence between Frisell and Joubert have been preserved.

Frederick William Wilson

He was the 2nd son of William Wilson, Manor House, Scarning, Norfolk and Elisa Turner, of Old

Great Gable

Among indirect ascents, a popular alternative is to climb Sour Milk Gill from Seathwaite in Borrowdale, first ascending Green Gable before traversing Windy Gap.

I treni di Tozeur

In 1981 Alice had won the San Remo song contest with another Battiato composition, "Per Elisa", which in turn both musically and lyrically was a paraphrase of Ludwig van Beethoven's bagatelle in A minor WoO 59, popularly known as "Für Elise".

Inma Cuesta

She is best known for her performance as Elisa Domínguez in Amar en tiempos revueltos.

José Eduardo Pinto da Costa

The eldest of six children of José Alexandrino Teixeira da Costa (Foz do Douro, Porto, June 9, 1910 – Aldoar, Porto, December 6, 1977), a Merchant, and wife (m. Cedofeita, Porto, November 15, 1932) Maria Elisa Bessa de Lima de Amorim Pinto (São Mamede de Infesta, Matosinhos, May 15, 1913 – November 14, 1997), one of his brothers is Jorge Nuno Pinto da Costa, the President of FC Porto.

Joseph Grossman

(William is said to have died as an indirect result of copying Harry Houdini's famous illusion featuring an escape from a tank of water.)

Kaspar Capparoni

He also works in various fiction television settings, which includes: the soap operas Ricominciamo (2000, TV miniseries), Piccolo mondo antico (TV miniseries), Incantesimo 4 (2001), Elisa di Rivombrosa (2003), La caccia (2005, a TV miniseries directed by Massimo Spano, as the antagonist for Alessio Boni), and for Capri (2006, TV series) alongside actress Bianca Guaccero.

KVI

Programs include "Sunrise Seattle", a Good Morning America-type program hosted by Mark Christopher and Elisa Jaffe, Clark Howard, Phil Hendrie, "The Buzz" with Scott Carty, the "Daily Wrap from the Wall Street Journal" with Michael Castner, ConsumerMan with Herb Weisbaum, and Don Imus, as well as paid programming on weekends.

Luca Paolini

As his name appeared in taped phone conversations together with Ivan Basso and his sister Elisa, he was suspected in illegal drugs usage.

Meg Giry

Elisa Heinsohn (opening cast), Catherine Ulissey, Tener Brown, Geralyn Del Corso, Jennifer Dawn Stillings, Joelle Gates, Heather McFadden, and Kara Klein in Broadway production of Andrew Lloyd Webber's musical.

Mitigated speech

Mitigated speech is a linguistic term describing deferential or indirect speech inherent in communication between individuals of perceived High Power Distance which has been in use for at least two decades with many published references.

Multicultural List

In February 1996 leader Athar Ali reacted strongly when a group of muslim city councilmen in Oslo took indirect distance to the Fatwā against novelist Salman Rushdie.

Paranormal Entity

Three indirect sequels were released, each one year after the other and also to coincide with the Paranormal Activity films, entitled 8213: Gacy House, Anneliese: The Exorcist Tapes and 100 Ghost Street: The Return of Richard Speck.

Pensacola and Atlantic Railroad

One indirect result of the building of the P&A was the development of the annual Chautauqua assemblies at Lake DeFuniak, near the town of the same name, which attracted thousands of visitors at the height of the Chautauqua movement, providing educational and cultural programs to residents and tourists.

Philip Battley

He is married to actress Elisa Boyd, is the nephew of television and film actor David Battley, the grandson of Labour MP John Battley,

Pollicipes polymerus

In a carefully designed series of experiments they recorded the direct and indirect results on the numbers of goose barnacles, sea mussels, acorn barnacles, starfish and predatory whelks (Nucella spp.) present in the area.

Pontus Fürstenberg

Without Pontus's direct and indirect patronage, artists like Carl Larsson, Ernst Josephson and Anders Zorn would not have been able to focus exclusively on their art.

Raymundo Joseph Peña

Born in Corpus Christi, Texas, he was the son of Cosme A. Peña and Elisa Ramon Peña.

Scots law

Although there was some indirect Roman law influence on Scots law, via the civil law and canon law used in the church courts, the direct influence of Roman law was slight up until around the mid-fifteenth century.

Sidney H. Liebson

At a time when electronics had not been able to make measurements with nanosecond accuracy, he developed several techniques to accomplish this accuracy for measuring organic fluorescence decay times and organic scintillation pulse widths by indirect means.

Silence on the Wire

Silence on the Wire: A Field Guide to Passive Reconnaissance and Indirect Attacks is a book by Michał Zalewski on some network-silent methods of information gathering as they relate to computer security.

Somnium Scipionis

Iain Pears wrote a historical novel called The Dream of Scipio which refers to Cicero's work in various direct and indirect ways.

Taya Zinkin

She was an indirect cause of the 1962 Border War between India and China for she discovered the Chinese building a road in Aksai Chin and she related it privately to Jawaharlal Nehru.

Varna culture

The discontinuity of the Varna, Karanovo, Vinča and Lengyel cultures in their main territories and the large scale population shifts to the north and northwest are indirect evidence of a catastrophe of such proportions that cannot be explained by possible climatic change, Desertification, or epidemics.

Victoria, Oriental Mindoro

Appeared in ABS-CBN Primetime Dramas and Shows, ASAP, MMK, Sabel, Angelito: Batang Ama, Nasaan Ka Elisa?