Queen Eleanor was present at the peace negotiations between Francis and Charles in Aigues-Mortes in 1538.
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He married Violante da Silva, daughter of João de Saldanha, Vedor of Queens Maria of Aragon and Eleanor of Austria, wives of King Manuel I of Portugal, and later of the Empress Isabella of Portugal, and wife Dona Joana de Lima of the Viscounts of Vila Nova de Cerveira.
He had letters of recommendation from James V to Eleanor of France, the Queen of Navarre, Madame Aubigny and Robert Stuart, sieur d'Aubigny, the Chancellor Antoine Duprat Cardinal of Sens, the Admiral Philippe de Chabot, the Grand Master Anne de Montmorency, and the French Secretary Jean le Breton, sieur de Villandry.