Interstate 39 and U.S. Route 51 provide access to the lake, as do several county highways.
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Buena Vista Marsh is located on fragmented lands south of the Village of Plover and the Wisconsin River, east of Biron, Wisconsin Rapids, and Kellner, north of State Road 73, Adams County, and Waushara County, and west of Bancroft and Interstate 39 / U.S. Highway 51.