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In March 1822, he returned to Rio in the company of scientists Édouard Ménétries (1802-1861), Ludwig Riedel (1761-1861), Christian Hasse and Nester Gaverilovitch Rubtsov (1799-1874), who would take care of zoological, botanical, astronomical and cartographical observations during the expedition.
The first to come was Ludwig Riedel (1761–1861), a German botanist who had participated in Baron von Langsdorff's famed expedition to Mato Grosso from 1826 to 1828.