The Restriction enzyme DpnI can recognize 5'-GmeATC-3'sites and digest the methylated DNA.
The biggest advantage of recombineering is that it obviates the need for conveniently positioned restriction sites, whereas in conventional genetic engineering, DNA modification is often compromised by the availability of unique restriction sites.
In the 1960s, it was shown in work done in the laboratories of Werner Arber and Matthew Meselson that the restriction is caused by an enzymatic cleavage of the phage DNA, and the enzyme involved was therefore termed a restriction enzyme.
enzyme | Registration, Evaluation, Authorisation and Restriction of Chemicals | Enzyme inhibition | enzyme inhibition | Restriction enzyme | Gonakudzingwa Restriction Camp | Enzyme inhibitor | enzyme inhibitor | Enzyme Commission number | Enzyme | Constitutive enzyme | Adaptive enzyme | Restriction digest | Phospholipase cleavage sites. Note that an enzyme that displays both PLA1 and PLA2 activities is called a ''Phospholipase B | Enzyme Records | Enzyme activator | DpnII restriction endonuclease family |