The party abandoned the colony to sail to the more prosperous colony of Santa María la Antigua del Darién, a colony established by the conquistador Vasco Núñez de Balboa without the knowledge of Nicuesa.
The Spanish founded San Sebastian de Uraba in 1509—abandoned within the year, and in 1510 the first permanent Spanish mainland settlement in America, Santa María la Antigua del Darién.
In September 1510, the first permanent European settlement, Santa María la Antigua del Darién on the Americas mainland was founded.
The historical site of Santa María la Antigua del Darién, the first European settlement built in the Americas mainland in 1510 by Alonso de Ojeda and destroyed in 1517 by the indios and therefore abandoned.
In 1510 Alonso de Ojeda founded San Sebastián de Urabá, the first Spanish settlement on the mainland, but that same year its provisional ruling, Francisco Pizarro, decided to leave and moved to a site in the Gulf of Urabá and founded under the direction of Martín Fernández de Enciso to Santa María la Antigua del Darién.
There is indirect evidence that the first permanent Spanish mainland settlement established in America was Santa María la Antigua del Darién.
There they founded Santa María la Antigua del Darién (c. 1509) and the now-vanished town of San Sebastian de Urabá (c. 1508-1510), the first two European settlements on the mainland of the Americas.
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Other provinces of this region during this era were Nueva Andalucia and Veragua or Castilla del Oro; the main city in Tierra Firme was Santa Maria La Antigua del Darién, now Darién, Panama, near at mouth of the Tarena river.
Between these limits lie Santa Maria La Antigua Del Darien on the Gulf of Urabá and Jurado on the Pacific side.