The player controls the movements of Professor Layton (voiced by Christopher Miller) and his young assistant Luke (voiced by Lani Minella in US English and Maria Darling in UK English) around the village of St. Mystere to locate the "Golden Apple" and solve other mysteries that arise during their search.
Rainer Maria Rilke | Carl Maria von Weber | Santa Maria | Maria Callas | Darling Downs | Santa Maria Capua Vetere | Maria | Maria Theresa | Henrietta Maria of France | José María Aznar | Maria Muldaur | Maria Bethânia | Carlo Maria Giulini | Basilica di Santa Maria Maggiore | Ave Maria | Santa Maria, Bulacan | Maria Theresa of Austria | Maria Edgeworth | Darling | Maria Montessori | María Conchita Alonso | Erich Maria Remarque | Darling River | Darling Harbour | Maria João | Maria Cantwell | Rainer Maria Latzke | Maria McKee | Alistair Darling | Santa María la Antigua del Darién |
As well being voiced by Rob Rackstraw and Jan Francis, the other characters are voiced by Maria Darling and Jimmy Hibbert.