The genome of H. volcanii consists of a large (4 Mb), multicopy chromosome and several megaplasmids.
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They translated the song "It's a Small World" into a series of DNA segments 150 base pairs long, inserted these into the bacteria, and were able to retrieve them without errors 100 bacterial generations later.
They are similar enough to be incorporated as part of DNA replication, but they possess side groups on the uracil component (an iodine and a CF3 group, respectively), that prevent base pairing.
James Michael Creeth (3 October 1924 – 15 January 2010) was an English biochemist whose experiments on DNA viscosity confirming the existence of hydrogen bonds between the purine and pyrimidine bases of DNA were crucial to Watson and Crick's discovery of the double helix structure of DNA.
The genome, consisting of 16,036 base pairs shows a large section of over 11,000 base pairs which are noncoding.
A 12 base pair insertion in the 5' flanking region of the Cystatin B gene has been linked to some individuals with Unverricht-Lundborg disease.
The middle 15 nucleotides are complementary to the target DNA or RNA and do not base pair with one another, while the five nucleotides at each terminus are complementary to each other rather than to the target DNA.