X-Nico

unusual facts about enzyme-linked immunosorbent assay



19-Norandrosterone

It is created as a byproduct of nandrolone via the 5-alpha reductase enzyme and is on the list of substances prohibited by the World Anti-Doping Agency since it is a detectable metabolite of nandrolone which is an anabolic steroid.

3,3',5,5'-Tetramethylbenzidine

3,3’,5,5’-Tetramethylbenzidine or TMB is a chromogenic substrate used in staining procedures in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays (ELISA).

ABTS

A common use for it is in the enzyme-linked immunosorbent assay (ELISA) to detect for binding of molecules to each other.

Allosteric enzyme

Allosteric enzymes are enzymes that change their conformational ensemble upon binding of an effector, which results in an apparent change in binding affinity at a different ligand binding site.

Aromadendrin

The enzyme dihydrokaempferol 4-reductase uses cis-3,4-leucopelargonidin and Artificial chemistry

Yasuhiro Suzuki has modeled various systems such as membrane systems, signaling pathways (P53), ecosystems, and enzyme systems by using his method, abstract rewriting system on multisets (ARMS).

ATP-binding motif

An ATP binding site is a protein micro-environment where ATP is captured and hydrolyzed to ADP, releasing energy that is utilized by the protein to "do work" by changing the protein shape and/or making the enzyme catalytically active.

Bacteriophage Qβ

The mechanism of lysis is similar to that of penicillin; A2 inhibits the formation of peptidoglycan by inhibiting the enzyme UDP-N-acetylglucosamine 1-carboxyvinyltransferase (MurA), which catalyzes the first committed step of peptidoglycan synthesis.

Beta-chamigrene synthase

The recombinant enzyme from the plant Arabidopsis thaliana produces (+)-alpha-barbatene, (+)-thujopsene and (+)-beta-chamigrene.

Bioanalysis

The most frequently used techniques are: liquid chromatography coupled with tandem mass spectrometry (LC–MS/MS) for 'small' molecules and enzyme-linked immunosorbent assay (ELISA) for macromolecules

Chimpanzee genome project

Cobalamin synthetase is a bacterial enzyme that makes Congenital adrenal hyperplasia due to 3 beta-hydroxysteroid dehydrogenase deficiency

3β-Hydroxysteroid dehydrogenase II deficient congenital adrenal hyperplasia (3β-HSD CAH) is an uncommon form of congenital adrenal hyperplasia (CAH) resulting from a mutation in the gene for one of the key enzymes in cortisol synthesis by the adrenal gland, 3β-hydroxysteroid dehydrogenase (3β-HSD) type II (HSD3B2).

CUTL1

Researchers from the Wellcome Trust Sanger Institute reported that the mutation of CUX1 reduces the inhibitory effects of a biological inhibitor, PIK3IP1 (phosphoinositide-3-kinase interacting protein 1), resulted in higher activity of the growth promoting enzyme, phosphoinositide 3-kinase (PI3K) which leads to tumor progression.

Delta11-fatty-acid desaturase

The enzyme from the marine microalga Thalassiosira pseudonana desaturates palmitic acid 16:0 to 16:1Delta11, whereas that from the leafroller moth Choristoneura rosaceana desaturates myristic acid 14:0 to 14:1Delta11.

Dihydrogossypetin

The enzyme Taxifolin 8-monooxygenase uses taxifolin, NADH, NADPH, H+, and O2 to produce 2,3-dihydrogossypetin, NAD+, NADP+, and H2O.

DNA polymerase delta

DNA polymerase delta is an enzyme complex found in eukaryotes that is involved in DNA replication and repair, and it consists of the proliferating cell nuclear antigen (PCNA), the multisubunit replication factor C, and the 4 subunit polymerase complex: POLD1, POLD2, POLD3, and POLD4.

Dopamine-responsive dystonia

A mutation in the gene GCH1, which encodes the enzyme GTP cyclohydrolase I, disrupts the production of BH4, decreasing dopamine levels (hypodopaminergia).

ENTPD2

It has been shown, by scientists from the University of Warwick, that E-NTPDase2 stimulates the growth of the eye: by testing the enzyme on tadpoles, the tadpoles were found to develop extra eyes on their body.

Flutamide

LH stimulates the testes to produce testosterone, which is metabolized to DHT by the enzyme 5α-reductase.

GLK

Glucokinase, an enzyme that facilitates the phosphorylation of glucose into glucose 6-phosphate.

Glyceride

Vegetable oils and animal fats contain mostly triglycerides, but are broken down by natural enzymes (lipases) into mono and diglycerides and free fatty acids.

GNPTG

The GNPTG gene codes instructions for making the gamma subunit of an enzyme called GlcNAc-1-phosphotransferase (also called N-acetylglucosamine-1-phosphate transferase).

Hydroxyquinol

Hydroxyquinol 1,2-dioxygenase is an enzyme that uses hydroxyquinol and O2 to produce 3-hydroxy-cis,cis-muconate.

Juniperus virginiana

Many grasses are C4 plants that concentrate CO2 levels in their bundle sheaths to increase the efficiency of RuBisCO, the enzyme responsible for photosynthesis, while Junipers are C3 plants that rely on (and may benefit from) the natural CO2 concentrations of the environment, although they are less efficient at fixing CO2 in general.

Lactose intolerance

The enzyme, β-galactosidase, is available in tablet form in a variety of doses, in many countries without a prescription.

Liver function tests

Lactate dehydrogenase (LDH) is an enzyme found in many body tissues, including the liver.

Long-chain 3-hydroxyacyl-coenzyme A dehydrogenase deficiency

Mutations in the HADHA gene lead to inadequate levels of an enzyme called long-chain 3-hydroxyacyl-coenzyme A (CoA) dehydrogenase, which is part of a protein complex known as mitochondrial trifunctional protein.

Maneb

Maneb interferes with glucocorticoid metabolism by inhibiting the activity of the enzyme 11β-hydroxysteroid dehydrogenase type 2 which converts cortisol to cortisone.

Maud Menten

In 1912 she moved to Berlin where she worked with Leonor Michaelis and co-authored their paper in Biochemische Zeitschrift (1913;49:333–369) which showed that the rate of an enzyme-catalyzed reaction is proportional to the amount of the enzyme-substrate complex.

Mixed-linkage glucan : Xyloglucan endotransglucosylase

Mixed-linkage glucan : xyloglucan endotransglucosylase (MXE) is a plant cell wall-modifying enzyme found in plants of the Equisetum genus.

Orotidine 5'-phosphate decarboxylase

This bifunctional enzyme is named UMP synthase and it also catalyzes the preceding reaction in pyrimidine nucleotide biosynthesis, the transfer of ribose 5-phosphate from 5-phosphoribosyl-1-pyrophosphate to orotate to form OMP.

Oxygenation

Oxygenase, an enzyme that oxidizes a substrate by transferring the oxygen from molecular oxygen O2 (as in air) to it

Peripheral chemoreceptors

However, AMPK is an enzyme found in many more types of cells than chemoreceptors because it helps regulate metabolism.

Phospholipase A

An enzyme that displays both phospholipase A1 and phospholipase A2 activities is called a Phospholipase B (see main article on phospholipases).

Phospholipase A1

Note that an enzyme that displays both PLA1 and PLA2 activities is called a Phospholipase B

Polyneuridine-aldehyde esterase

This enzyme is found in various forms in plant species such as Arabidopsis thaliana, Glycine max (soybean), Vitis vinifera (wine grape), and Solanum lycopersicum (tomato) among others.

Polynucleotide phosphorylase

The same abbreviation (PNPase) is also used for another, otherwise unrelated enzyme, Purine nucleoside phosphorylase.

Pyocyanine

Firstly, the NADPH used by pyocyanin depletes the available substrate for the reaction catalysed by the NADPH oxidase enzyme.

Renato Dulbecco

As demonstrated by Temin and Baltimore, who shared the Nobel Prize with Dulbecco, the transfer of viral genes to the cell is mediated by an enzyme called reverse transcriptase (or, more precisely, RNA-dependent DNA polymerase), which replicates the viral genome (in this case made of RNA) into DNA, which is later incorporated in the host genome.

Resazurin

Usually, in the presence of NADPH dehydrogenase or NADH dehydrogenase as the enzyme, NADPH or NADH is the reductant that converts resazurin to resorufin.

Shugoshin N terminal protein domain

Shugoshin protein is thought to act by protecting two proteins, named Rec8 and Rad21 at the centromeres from protein degradation by the enzyme, separase.

Sipco Industries

A world pioneer in enzyme technology, Sipco is best known for helping the United States Postal Service (USPS) with Anthrax following the attacks on the US Senate shortly after September 11, 2001.

System size expansion

The corrections have been shown to be particularly considerable for allosteric and non-allosteric enzyme-mediated reactions in intracellular compartments.

Transaldolase deficiency

Transaldolase deficiency is a disease characterised by abnormally low levels of the Transaldolase enzyme.

Direct sequence analysis of genomic DNA from blood can be used to perform a mutation analysis for the TALDO1 gene responsible for the Transaldolase enzyme.

Transition state theory

Purine nucleoside phosphorylase (PNP) is an enzyme involved in the catabolism and recycling of nucleosides and is a target for the development of novel therapeutic agents for T-cell apoptosis in leukemia and in autoimmune diseases.

Transsulfuration pathway

All four transsulfuration enzymes are PLP enzymes and all bar Cystathionine γ-synthase are members of the Cys/Met metabolism PLP-dependent enzyme family (type I PLP enzymes).

Very long-chain acyl-coenzyme A dehydrogenase deficiency

Mutations in the ACADVL gene lead to inadequate levels of an enzyme called very long-chain acyl-coenzyme A (CoA) dehydrogenase.

Virus quantification

ELISA is a more modern variation of a protein assay that utilizes a specific antibody linked to an enzyme to detect the presence of an unknown amount of antigen (i.e. virus) in a sample.


see also

Elisa

ELISA or Enzyme-Linked Immunosorbent Assay, a biochemical technique

Tapinoma sessile

They labeled sucrose with Immunoglobin G (IgG) proteins, and then identified them using an enzyme-linked immunosorbent assay (ELISA) to track the movement of food.