X-Nico

23 unusual facts about 16S ribosomal RNA


'The All-Species Living Tree' Project

'The All-Species Living Tree' Project is a collaboration between various academic groups/institutes, such as ARB, SILVA rRNA database project, and LPSN, with the aim of assembling a database of 16S rRNA sequences of all validly published species of Bacteria and Archaea.

Archaeal Richmond Mine acidophilic nanoorganisms

These novel groups of archaea named ARMAN-1, ARMAN-2 (Candidatus Micrarchaeum acidiphilum ARMAN-2 ), and ARMAN-3 were missed by previous PCR-based surveys of the mine community because the ARMANs have several mismatches with commonly used PCR primers for 16S rRNA genes.

Archaellum

Archaea were first classified as a separate group of prokaryotes in 1977 by Carl Woese and George E. Fox based on the differences in the sequence of ribosomal RNA (16S rRNA) genes.

Bacillus megaterium

Phylogenetically, based on 16S rRNA, B. megaterium is strongly linked with B. flexus, the latter distinguished from B. megaterium a century ago, but only recently confirmed as a different species.

Candidate division

A candidate division, candidate phylum or candidate division-level is a lineage of Prokaryotic organisms for which no cultured representatives have been found, but evidence of the existence of the clade has been obtained by 16S rRNA metagenomic analysis of environmental samples.

Candidate division TM7

The candidate division TM7, is a major lineage of Bacteria, the existence of which is known solely through environmental 16S rRNA sequences as to date no species has been grown in the lab, a requirement for taxonomy, making it a candidate phylum.

Candidatus Liberibacter

Detection of the liberibacters is based on PCR amplification of their 16S rRNA gene with specific primers.

Cronobacter sakazakii

The taxonomic relationship between E. sakazakii strains has been studied using full-length 16S rRNA gene sequencing, DNA-DNA hybridization, multilocus sequence typing (MLST), f-AFLP, automated ribotyping.

Earth Microbiome Project

The 16S ribosomal RNA gene for bacteria and the 18S ribosomal RNA gene for protists are often used as target genes for this purpose.

Eocypraeidae

has raised the tribe Eocypraeini to the status of the family Eocypraeidae, based on morphological research of the radulae, shell and animal morphology and molecular phylogeny research of the 16S ribosomal RNA gene.

EzTaxon Database

EzTaxon is a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences.

Hoff crab

Phylogenetic analysis of the mitochondrial 16S ribosomal RNA gene and a range of other anomuran crustaceans, using Bayesian inference, places this species from the East Scotia Ridge as a sister taxon to Kiwa hirsuta, with a sequence divergence from this species of 6.45%, which is consistent for within-genus divergence in squat lobsters.

Human Microbiome Project

The project also is financing deep sequencing of bacterial 16S rRNA sequences amplified by polymerase chain reaction from human subjects.

The microbial genome data were extracted by identifying the bacterial specific ribosomal RNA, 16S rRNA.

Lacy elimia

In a recent genetic sequence study of the 16S ribosomal RNA gene, the lacy elimia was found to be very similar to the compact elimia (Elimia showalteri).

Mycobacterium abscessus

M. abscessus and M. chelonae sequevar I share an identical sequence in the 54-510 region of 16S rRNA, though both species can be differentiated by their hsp65, ITS or rpoB gene sequences.

Neisseria bacilliformis

16S rRNA phylogenetic analysis has been used to identify a member of Neisseria from closely related genera, but this method is unable to distinguish amongst the various species that compose the genus Neisseria.

Ruminococcus

As usual, bacteria taxonomy is in flux, with Clostridia being paraphyletic, and some erroneous members of Ruminococcus being reassigned to a new genus Blautia on the basis of 16S rRNA gene sequences.

Shewanella violacea

These categories were created from an evaluation of the 16s rRNA sequences as well as a comparison of membrane lipid compositions.

Staphylococcus devriesei

It was originally isolated from cow's milk and teats and, on the basis of 16S ribosomal RNA sequence, is most genetically similar to Staphylococcus haemolyticus, S. hominis, and S. lugdunensis.

Staphylococcus saccharolyticus

The species was formerly known as Peptococcus saccharolyticus but was reclassified on the basis of 16S ribosomal RNA and biochemical similarity to other members of Staphylococcus.

Thermoanaerobacter kivui

However, further 16S ribosomal RNA studies put the bacteria into genus Thermoanaerobacter and the genus was no longer necessary.

Thermococcus kodakarensis

The isolate was originally named Pyrococcus kodakarensis KOD1, but reclassified as a species of Thermococcus, based on 16S rRNA sequence.