X-Nico

5 unusual facts about RNA-Seq


DNASTAR

The latest version of ArrayStar, version 5.0, which was released in 2011, also includes an optional module, QSeq, to allow scientists to visualize and analyze RNA-Seq and ChIP-Seq data using the same tools as are used for microarray gene expression.

Jörg Vogel

Among other achievements he pioneered the application of RNA-Seq for the analysis of the bacterial transcription.

MochiView

The software uses a generalized data format that serves other purposes as well, such as the visualization and analysis of RNA-Seq data or the import, maintenance, exploration, and analysis of Sequence motif libraries.

RNA-Seq

Weighted gene co-expression network analysis has been successfully used to identify co-expression modules and intramodular hub genes based on RNA seq data.

There are many tools available for aligning genomic reads to a reference genome (sequence alignment tools), however, special attention is needed when alignment of a transcriptome to a genome, mainly when dealing with genes having intronic regions.


Abelson murine leukemia virus

As a retrovirus, it has a single-stranded, positive sense RNA genome which replicates via a DNA intermediate mediated by a reverse transcriptase.

Abraham Firkovich

Further exposures were made by Strack and Harkavy (St. Petersburg, 1875) in the Catalog der Hebr. Bibelhandschriften der Kaiserlichen Oeffentlichen Bibliothek in St. Petersburg; in Harkavy's Altjüdische Denkmäler aus der Krim (ib. 1876); in Strack's A. Firkowitsch und Seine Entdeckungen (Leipsic, 1876); in Fränkel's Aḥare Reshet le-Baḳḳer (Ha-Shaḥar, vii. 646 et seq.); in Deinard's Massa' Ḳrim (Warsaw, 1878); and in other places.

AfaR small RNA

It is an Hfq-dependent RNA which downregulates AfaD-VIII invasin translation by binding to and initiating cleavage of its mRNA.

Alkyl phosphate

For example, high energy metabolites such as ATP and PEP are alkyl phosphates, as are nucleic acids such as DNA and RNA.

Antizyme RNA frameshifting stimulation element

Antizyme RNA frameshifting stimulation element is a structural element which is found in antizyme mRNA and is known to promote frameshifting.

Archaellum

Archaea were first classified as a separate group of prokaryotes in 1977 by Carl Woese and George E. Fox based on the differences in the sequence of ribosomal RNA (16S rRNA) genes.

Brevig Mission, Alaska

In the late 1990s, a team of scientists led by Johan Hultin exhumed the body of an Inuit woman who had been buried in the permafrost in a gravesite near Brevig Mission in an attempt to recover RNA of the 1918 influenza virus (Spanish flu) that killed her.

Bulbul coronavirus HKU11

Bulbul coronavirus HKU11 is a single-stranded, positive-sense RNA deltacoronavirus of avian origin found in Chinese Bulbuls.

Caulimoviridae

As replication requires the use of RNA intermediate, viruses from the Caulimoviridae family are not true dsDNA viruses - instead they are termed DNA reverse-transcribing viruses.

Compounds of oxygen

Oxygen also occurs in phosphate (PO43−) groups in the biologically important energy-carrying molecules ATP and ADP, in the backbone and the purines (except adenine) and pyrimidines of RNA and DNA, and in bones as calcium phosphate and hydroxylapatite.

David Baulcombe

In 1998 Craig Mello and Andrew Fire reported a potent gene silencing effect after injecting double stranded RNA into C. elegans.

DNA Analysis Backlog Elimination Act of 2000

The DNA Analysis Backlog Elimination Act of 2000 (H.R. 4640, 42 U.S.C. 14135 et seq.) is a United States Act of Congress that primarily allows U.S. States to carry out DNA analyses for use in the FBI's Combined DNA Index System and to collect and analyse DNA samples.

Ekka

The Ekka is organised by the Royal National Agricultural and Industrial Association of Queensland (RNA), and is held at the RNA Showgrounds in the inner-city suburb of Bowen Hills approximately two kilometres north-east of Brisbane's city centre.

Evolution in Variable Environment

EVE operates under the “central dogma,” the assumption that all biochemical pathways proceed through the following steps: DNA => RNA => protein.

FAIRE-Seq

FAIRE-seq data are mapped to the human genome assembly and displayed as part of the ENCODE project at the UCSC Genome Browser.

Gene silencing

Sidney Altman and Thomas Cech first discovered catalytic RNA molecules, RNase P and group II intron ribozymes, in 1989 and won the Nobel Prize for their discovery.

General feature format

The general feature format (gene-finding format, generic feature format, GFF) is a file format used for describing genes and other features of DNA, RNA and protein sequences.

Hasideans

The piety attributed to Ḥasidim in the Talmudic sources is not in any way abnormal or suggestive of sect (Lehmann, in R. E. J. xxx. 182 et seq.).

History of religious Jewish music

Forkel, All-gemeine Gesch. der Musik. i. 173 et seq. and the bibliography there given.E.

J. Heinrich Matthaei

Whilst a post-doctoral visitor in the laboratory of Marshall Warren Nirenberg at the NIH in Bethesda, Maryland, he discovered that a synthetic RNA polynucleotide, composed of a repeating uridylic acid residue, coded for a polypeptide chain encoding just one kind of amino acid, phenylalanine.

John Abelson

His work has made possible an understanding of how genomic DNA can be converted to both messenger RNA and transfer RNA, particularly when there are introns present in the genome.

Lacto-rpoB RNA motif

These genes encode a subunit of RNA polymerase, and it is hypothesized that Lacto-rpoB RNA participate in the regulation of these genes.

Listeria Hfq binding LhrA

Listeria Hfq binding LhrA is a ncRNA that was identified by screening for RNA molecules which co-immunoprecipitated with the RNA chaperone Hfq.

Memory RNA

Memory RNA is a now-discredited hypothetical form of RNA that was proposed by James V. McConnell and others as a means of explaining how long-term memories were stored in the brain.

The basic principle or the memory RNA was also used by comic book writer Alan Moore to explain the origin of DC Comics' character the Swamp Thing in Saga of the Swamp Thing #21.

National Do Not Call Registry

On June 27, 2003, the U.S. Federal Trade Commission (FTC) opened the National Do Not Call Registry in order to comply with the Do-Not-Call Implementation Act of 2003 (Public Law No. 108-10, was H.R. 395 of the 108th Congress, and codified at 15 U.S.C. § 6101 et. seq.), sponsored by Representatives Billy Tauzin and John Dingell and signed into law by President George W. Bush on March 11, 2003.

Nelson Bay virus

Nelson Bay virus (NBV)is a novel double-stranded RNA orthoreovirus species first isolated from a flying fox (Pteropus poliocephalus) near Nelson Bay in New South Wales, Australia.

Norovirus cis-acting replication element

In molecular biology, the Norovirus cis-acting replication element (CRE) is an RNA element which is found in the coding region of the RNA-dependent RNA polymerase in Norovirus.

Nothing to Hide

She tells her friend to leave, scaring Tina who believes that Niki has turned into a completely different person, an assumption which is confirmed by the appearance of the RNA symbol tattoo on Niki's shoulder, showing that Jessica is in control.

R1 plasmid

CopA-like RNA, an antisense RNA involved in replication control of the plasmid.

R43

Small nucleolar RNA R43, a plant non-coding RNA molecule which functions in the modification of other small nuclear RNAs

Renato Dulbecco

As demonstrated by Temin and Baltimore, who shared the Nobel Prize with Dulbecco, the transfer of viral genes to the cell is mediated by an enzyme called reverse transcriptase (or, more precisely, RNA-dependent DNA polymerase), which replicates the viral genome (in this case made of RNA) into DNA, which is later incorporated in the host genome.

Richard A. Jorgensen

His and Carolyn Napoli's observations of pigment gene 'cosuppression' in Petunia flowers are examples of post transcriptional gene silencing that predated the discovery of RNA interference (RNAi) and contributed to the current understanding of the commonality of RNA-mediated gene silencing in eukaryotes.

RN5S1@

RNA, 5S cluster 1, also known as RN5S1@, is a human gene encoding the 5S subunit of ribosomal RNA.

SERBP1

Plasminogen activator inhibitor 1 RNA-binding protein (serbp1, not to be confused with srebp1) is a protein that in humans is encoded by the SERBP1 gene.

Shir ha-Shirim Rabbah

Some scholars (I.H. Weiss, Dor iii. 263-264; and Adolf Jellinek, in a letter to Theodor, reprinted in Monatsschrift, 1879, pp. 237 et seq.), moreover, have assumed a direct connection between such ancient discourses and the present Canticles Rabbah, regarding this midrash as an old collection of these discourses, increased by various later additions.

Small Cajal body specific RNA 17

Small Cajal body-specific RNA 17 (also known as U12-22 scaRNA) is a type of small nuclear RNA which localises to the cajal bodies and proposed to guide the modification of RNA polymerase II transcribed spliceosomal RNAs U1, U2, U4, U5 and U12.

Small Cajal body specific RNA 18

small Cajal body-specific RNA 18 (also known as U91 or U4-8) is a type of small nuclear RNA which localises to the cajal bodies and proposed to guide the modification of RNA polymerase II transcribed spliceosomal RNAs U1, U2, U4, U5 and U12.

Spectrophotometer for Nucleic Acid Measurements

Analysis of nucleic acids is commonly performed to determine the average concentrations of DNA or RNA present in a mixture, as well as their purity.

Surface-enhanced Raman spectroscopy

SERS can be used to target specific DNA and RNA sequences using a combination of gold and silver nanoparticles and Raman-active dyes, such as Cy3.

Totiviridae

It contains 2 overlapping open reading frames (ORF)—gag and pol—which respectively encode the capsid protein and the RNA-dependent RNA polymerase.

Totivirus

These open reading frames (ORFs) code for a capsid protein (CP) and an RNA-dependent RNA polymerase (RDRP).

Transcriptional regulation

General transcription factors These transcription factors position RNA polymerase at the start of a protein-coding sequence and then release the polymerase to transcribe the mRNA.

Trichloroacetic acid

It is widely used in biochemistry for the precipitation of macromolecules, such as proteins, DNA, and RNA.

Vibrio cholerae ToxT activated RNAs

Regulation of PtsG by TarA may be dependent upon the Hfq protein, an RNA chaperone.

Vibrio regulatory RNA of OmpA

VrrA (Vibrio regulatory RNA of OmpA) is a non-coding RNA that is conserved across all Vibrio species of bacteria and acts as a repressor for the synthesis of the outer membrane protein OmpA.

Z12

Z12 small nucleolar RNA, a non-coding RNA molecule which functions in the modification of other small nuclear RNAs


see also